The KO Validated Interleukin-18 Polyclonal Antibody (CAB1115) is an essential tool for researchers studying interleukin-18 (IL-18), a proinflammatory cytokine involved in immune responses. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been rigorously validated for Western blot applications. By binding specifically to IL-18, it allows for accurate detection and analysis in various cell types, making it an ideal choice for studies in immunology and inflammatory diseases.
IL-18 is a key mediator of inflammation and plays a crucial role in various immune responses, including the regulation of T cell activation and cytokine production. Dysregulation of IL-18 has been implicated in several inflammatory conditions, making it a promising target for therapeutic intervention. By using the KO Validated Interleukin-18 Polyclonal Antibody, researchers can gain valuable insights into the role of IL-18 in disease pathology and potentially identify novel strategies for treatment.
Product Name:
[KO Validated] IL18 Rabbit Polyclonal Antibody
SKU:
CAB1115
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 37-193 of human IL18 (NP_001553.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
IGIF; IL-18; IL-1g; IL1F4; IL18
Positive Sample:
Recombinant Human IL-18 Protein
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
22kDa
Observed MW:
18kDa
The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3606
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of recombinant Human IL-18 Protein using IL18 Rabbit pAb (CAB1115) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 10 ng/1 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
