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[KO Validated] Insulin-degrading enzyme (IDE) Rabbit Polyclonal Antibody (CAB1630)

SKU:
CAB1630
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • KO Validated Antibodies 1 Anti-IDE Antibody CAB1630KO Validated
  • KO Validated Antibodies 1 Anti-IDE Antibody CAB1630KO Validated
  • KO Validated Antibodies 1 Anti-IDE Antibody CAB1630KO Validated
$167 - $503
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Description

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Product Name:[KO Validated] Insulin-degrading enzyme (IDE) Rabbit Polyclonal Antibody
SKU:CAB1630
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) (NP_004960.2).
Sequence:MRYR LAWL LHPA LPST FRSV LGAR LPPP ERLC GFQK KTYS KMNN PAIK RIGN HITK SPED KREY RGLE LANG IKVL LISD PTTD KSSA ALDV HIGS LSDP PNIA GLSH FCEH MLFL GTKK YPKE NEYS QFLS EHAG SSNA FTSG EHTN YYFD VSHE HLEG ALDR FAQF FLCP LFDE SCKD REVN AVDS EHEK NVMN DAWR LFQL EKAT GNPK HPFS KFGT GNKY TLET RPNQ EGID VRQE LLKF HSAY YS
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:10 - 1:100
Synonyms:INSULYSIN; E)
Positive Sample:DU145,Mouse liver
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Secreted.
Calculated MW:118kDa
Observed MW:118kDa

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.

Purification Method:Affinity purification
Gene ID:3416
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) antibody (CAB1630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).

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