The IDE Polyclonal Antibody (CAB1630) is a reliable tool for researchers studying the IDE protein, which plays a crucial role in regulating insulin and amyloid beta levels in the body. This antibody, generated in rabbits, is specifically designed for detecting and analyzing IDE in human samples, making it an essential component for studies in diabetes, Alzheimer's disease, and other metabolic disorders.IDE, also known as insulin-degrading enzyme, is a key player in the degradation of insulin and other peptides, contributing to the maintenance of glucose homeostasis and the clearance of amyloid beta, a toxic protein implicated in Alzheimer's disease.
Understanding the role of IDE in these processes is essential for developing therapeutic strategies targeting insulin resistance, diabetes, and neurodegenerative diseases.The IDE Polyclonal Antibody is validated for use in Western blot applications, providing researchers with a sensitive and specific tool for studying IDE expression and activity in various cell types and tissues. Its high reactivity and specificity make it an ideal choice for investigating the mechanisms underlying IDE function and its potential as a therapeutic target in metabolic and neurodegenerative disorders.
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) (NP_004960.2).
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
Purification Method:
Affinity purification
Gene ID:
3416
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE)) antibody (CAB1630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).
