The HPRT1 Polyclonal Antibody (CAB5692) is a valuable tool for researchers studying the HPRT1 gene, which encodes the enzyme hypoxanthine phosphoribosyltransferase 1. This enzyme plays a crucial role in the salvage pathway of purine synthesis, making it essential for DNA replication and repair in cells. Raised in rabbits, this antibody demonstrates high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the HPRT1 protein, enabling accurate detection and analysis in various cell types.
The HPRT1 gene is implicated in various genetic disorders, including Lesch-Nyhan syndrome, which is characterized by neurological and behavioral abnormalities. Additionally, mutations in HPRT1 have been linked to certain forms of cancer, highlighting its significance in disease research. By using the HPRT1 Polyclonal Antibody, researchers can gain valuable insights into the function and regulation of this important enzyme, paving the way for advancements in medical research and potential therapeutic strategies for related disorders.
Product Name:
[KO Validated] HPRT1 Rabbit Polyclonal Antibody
SKU:
CAB5692
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Purification Method:
Affinity purification
Gene ID:
3251
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] HPRT1 Rabbit pAb (CAB5692) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
