The HNRNPA1 Polyclonal Antibody (CAB18094) is a valuable tool for researchers studying HNRNPA1, a key RNA binding protein involved in various cellular processes. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the HNRNPA1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it a versatile tool for studies in molecular biology and RNA processing research.
HNRNPA1 is known for its role in regulating alternative splicing, mRNA stability, and translation, making it essential for proper gene expression and cellular function. Dysregulation of HNRNPA1 has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance in disease progression. Understanding the functions of HNRNPA1 is crucial for unraveling the molecular mechanisms underlying these conditions and developing targeted therapies in the future.
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.
Purification Method:
Affinity purification
Gene ID:
3178
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and hnRNP A1 knockout (KO) 293T cells, using [KO Validated] hnRNP A1 Rabbit pAb (CAB18094) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
