The KO-Validated HIF1AN/FIH1 Rabbit Monoclonal Antibody (CAB3701) is a valuable tool for researchers studying the hypoxia-inducible factor 1 alpha inhibitor (HIF1AN) protein. This antibody, which is produced in rabbits, has been rigorously validated for knockout (KO) validation and is highly specific for detecting HIF1AN/FIH1 in various experimental settings.HIF1AN/FIH1 is a key regulator of the cellular response to hypoxia, playing a critical role in the stability and activity of HIF-1 alpha, a master transcription factor involved in the response to low oxygen levels.
Dysregulation of HIF1AN/FIH1 has been implicated in a variety of diseases, including cancer, cardiovascular disease, and neurodegenerative disorders.With its high specificity and sensitivity, the CAB3701 antibody is ideal for Western blotting, immunofluorescence, and immunoprecipitation studies aimed at understanding the function and regulation of HIF1AN/FIH1. By utilizing this antibody, researchers can gain valuable insights into the role of HIF1AN/FIH1 in cellular responses to hypoxia and its potential as a therapeutic target in various disease states.
Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.
Purification Method:
Affinity purification
Gene ID:
55662
Clone Number:
ARC0826
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts from wild type (WT) and HIF1AN/FIH1 knockout (KO) 293T cells, using HIF1AN/FIH1 antibody (CAB3701) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
