Anti-HERC4 Antibody (CAB18049)[KO Validated]
- SKU:
- CAB18049
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
Anti-HERC4 Antibody (CAB18049)[KO Validated]
The HERC4 Polyclonal Antibody (CAB18049) is specifically designed for research involving HERC4, a protein belonging to the HERC family of E3 ubiquitin ligases. This antibody, raised in rabbits, is highly reactive with rabbit samples and has been validated for use in various applications such as Western blotting and immunohistochemistry.HERC4 is known to play a crucial role in regulating protein degradation and turnover through the ubiquitin-proteasome system. Its involvement in various cellular processes, including DNA repair and immune response, makes it an attractive target for studying diseases such as cancer and neurodegenerative disorders.
This HERC4 Polyclonal Antibody enables the detection and analysis of HERC4 protein expression in different cell types and tissues, providing researchers with a valuable tool for investigating the functional roles of HERC4 in cellular pathways and disease development. Whether studying protein degradation mechanisms or exploring new therapeutic targets, this antibody is essential for advancing our understanding of HERC4 biology.
| Antibody Name: | Anti-HERC4 Antibody [KO Validated] |
| Antibody SKU: | CAB18049 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3. |
| Isotype: | IgG |
| Sequence: | WTVN EALI QKWL SYPS GRFP VEIA NEID GTFS SSGC LNGS FLAV SNDD HYRT GTRF SGVD MNAA RLLF HKLI QPDH PQIS QQVA ASLE KNLI PKLT SSLP DVEA LRFY LTLP ECPL MSDS NNFT TIAI PFGT ALVN LEKA PLKV LENW WSVL EPPL FLKI VELF KEVV VHLL KLYK IGIP PSER RIFN SFLH TALK VLEI LHRV NEKM GQII QYDK FYIH EVQE LIDI RNDY INWV QQQA YGML ADIP VTIC TYPF VFDA QAKT TLLQ TDAV LQMQ MAID QAHR QNVS SLFL PVIE SVNP C |
| Gene ID: | 26091 |
| Uniprot: | Q5GLZ8 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 12kDa/13kDa/106kDa/109kDa/117kDa/118kDa |
| Observed MW: | 118kDa |
![Anti-HERC4 Antibody [KO Validated] (CAB18049)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/s/049/A18049_1__44683.jpg) | Western blot analysis of extracts from normal (control) and HERC4 knockout (KO) HeLa cells, using HERC4 antibody (CAB18049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min. |
| Synonyms: | HERC4 |
| Background: |
| UniProt Protein Function: | HERC4: Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.- Chromosomal Location of Human Ortholog: 10q21.3 Cellular Component: cytoplasm; nucleus; cytosol Molecular Function:ubiquitin-protein ligase activity; ligase activity Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; spermatogenesis; cell differentiation |
| NCBI Summary: | HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q5GLZ8 |
| NCBI GenInfo Identifier: | 74707832 |
| NCBI Gene ID: | 26091 |
| NCBI Accession: | Q5GLZ8.1 |
| UniProt Secondary Accession: | Q5GLZ8,Q5GC98, Q5GC99, Q5GCA0, Q8IXP9, Q9HCH9, |
| UniProt Related Accession: | Q5GLZ8 |
| Molecular Weight: | 1057 |
| NCBI Full Name: | Probable E3 ubiquitin-protein ligase HERC4 |
| NCBI Synonym Full Names: | HECT and RLD domain containing E3 ubiquitin protein ligase 4 |
| NCBI Official Symbol: | HERC4Â Â |
| NCBI Protein Information: | probable E3 ubiquitin-protein ligase HERC4; hect domain and RLD 4; HECT domain and RCC1-like domain-containing protein 4 |
| UniProt Protein Name: | Probable E3 ubiquitin-protein ligase HERC4 |
| UniProt Synonym Protein Names: | HECT domain and RCC1-like domain-containing protein 4 |
| Protein Family: | Probable E3 ubiquitin-protein ligase |
| UniProt Gene Name: | HERC4Â Â |
| UniProt Entry Name: | HERC4_HUMAN |
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