The KO Validated HADHA Polyclonal Antibody (CAB5346) is a valuable tool for researchers studying the HADHA protein, which plays a critical role in fatty acid metabolism and energy production within the mitochondria. This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications.HADHA, also known as Trifunctional enzyme subunit alpha, is essential for the beta-oxidation of long-chain fatty acids and helps maintain energy balance in cells. Dysregulation of HADHA has been linked to metabolic disorders such as fatty acid oxidation disorders and mitochondrial diseases.
By targeting the HADHA protein, researchers can gain insights into the mechanisms of these conditions and potentially develop new therapeutic strategies.Whether investigating the role of HADHA in metabolic pathways, mitochondrial function, or disease progression, the KO Validated HADHA Polyclonal Antibody offers a reliable tool for detecting and analyzing this crucial protein in various cell types. Its validation for knockout studies ensures accurate and specific results, enabling researchers to advance their understanding of HADHA's functions and implications in health and disease.
Product Name:
[KO Validated] HADHA Rabbit Polyclonal Antibody
SKU:
CAB5346
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).
WB,1:2000 - 1:6000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA; HA
Positive Sample:
293T,K-562
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
83kDa
Observed MW:
78kDa
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Purification Method:
Affinity purification
Gene ID:
3030
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using [KO Validated] HADHA Rabbit pAb (CAB5346) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
