The GSS Polyclonal Antibody (CAB14535) is a valuable tool for researchers studying GSS, a key enzyme involved in various cellular processes such as signal transduction and cell proliferation. This antibody, derived from rabbit sources, has high specificity for GSS in human samples and has been validated for Western blot applications.GSS, also known as glutathione synthetase, plays a crucial role in the synthesis of the antioxidant glutathione, which protects cells from oxidative stress and maintains cellular homeostasis. Dysregulation of GSS activity has been implicated in diseases such as cancer, neurodegenerative disorders, and metabolic diseases, making it an important target for therapeutic intervention.
By using the GSS Polyclonal Antibody, researchers can detect and analyze GSS protein levels in various cell types, allowing for a better understanding of its role in disease pathogenesis and potential therapeutic strategies. This antibody is a valuable tool for studies in biochemistry, molecular biology, and drug discovery related to GSS function and regulation.
Recombinant fusion protein containing a sequence corresponding to amino acids 373-474 of human Glutathione Synthetase (Glutathione Synthetase (GSS)) (NP_000169.1).
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Purification Method:
Affinity purification
Gene ID:
2937
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Glutathione Synthetase (Glutathione Synthetase (GSS)) knockout (KO) 293T cells, using [KO Validated] Glutathione Synthetase (GSS) Rabbit pAb (CAB14535) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
