The KO Validated FUS Polyclonal Antibody (CAB5921) is a powerful tool for researchers studying FUS, a key player in RNA processing and neurodegenerative diseases. This antibody, produced in rabbits, has been rigorously tested and validated for knockout validation, ensuring reliable results in various experimental settings.FUS, also known as fused in sarcoma, is a multifunctional RNA-binding protein involved in mRNA splicing, transcriptional regulation, and DNA repair. Dysregulation of FUS has been linked to diseases like amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), making it a promising target for therapeutic intervention.
With its high specificity and sensitivity in detecting FUS protein, this antibody is an indispensable tool for researchers investigating the roles of FUS in normal physiological processes and disease pathology. Its applications in techniques such as immunofluorescence, immunoprecipitation, and flow cytometry make it versatile for a wide range of studies in neuroscience and molecular biology. Unlock the potential of FUS research with the KO Validated FUS Polyclonal Antibody.
Product Name:
[KO Validated] FUS Rabbit Polyclonal Antibody
SKU:
CAB5921
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 297-526 of human FUS (NP_004951.1).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TLS; ALS6; ETM4; FUS1; POMP75; altFUS; HNRNPP2; US
Positive Sample:
U-937,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
70kDa
This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.
Purification Method:
Affinity purification
Gene ID:
2521
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and FUS knockout (KO) 293T cells, using [KO Validated] FUS Rabbit pAb (CAB5921) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
