The KO Validated ETFA Polyclonal Antibody (CAB19937) is a valuable tool for researchers studying ETFA, a key protein involved in electron transfer processes in the mitochondria. Produced in rabbits, this antibody is highly specific and reacts well with human samples, making it ideal for use in Western blot applications. By targeting the ETFA protein, this antibody allows for the detection and analysis of ETFA in various cell types, providing valuable insights for studies in bioenergetics and metabolic disorders.ETFA is a critical component of the electron transfer flavoprotein complex, playing a crucial role in the mitochondrial beta-oxidation pathway.
Dysregulation of ETFA has been linked to metabolic diseases, making it a potential target for therapeutic interventions. Research on ETFA is essential for understanding mitochondrial function and energy metabolism, with implications for diseases such as diabetes, obesity, and fatty acid oxidation disorders. The KO Validated ETFA Polyclonal Antibody (CAB19937) provides researchers with a reliable tool to further investigate the functions and dysregulation of ETFA in these contexts.
Product Name:
[KO Validated] ETFA Rabbit Polyclonal Antibody
SKU:
CAB19937
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1).
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit pAb (CAB19937) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
