The EMD Polyclonal Antibody (CAB18030) is a highly specific and validated antibody designed for research involving EMD, a protein involved in various cellular processes including DNA repair, transcription, and nuclear envelope organization. This antibody, raised in rabbits, is suitable for use in a variety of applications, including Western blot and immunofluorescence.EMD, also known as emerin, is a key component of the nuclear envelope and has been linked to several genetic disorders including Emery-Dreifuss muscular dystrophy.
Research on EMD is essential for understanding its role in cellular function and disease pathology. The EMD Polyclonal Antibody is an invaluable tool for investigating the expression and localization of EMD in different cell types and tissues, making it ideal for studies in cell biology, genetics, and developmental biology.
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Purification Method:
Affinity purification
Gene ID:
2010
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Emerin/Emerin/EMD knockout (KO) 293T cells, using [KO Validated] Emerin/EMD Rabbit pAb (CAB18030) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
