The EMD Polyclonal Antibody (CAB18016) is specifically designed for research involving EMD, a cell membrane protein associated with cell proliferation, migration, and differentiation. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. EMD, also known as Emerin, is a key player in nuclear envelope stability and is involved in various cellular processes such as gene expression regulation and signal transduction. Dysregulation of EMD expression has been linked to diseases such as Emery-Dreifuss muscular dystrophy and certain cancers, making it a valuable target for research in molecular biology and disease pathogenesis.
By binding specifically to the EMD protein, this antibody enables precise detection and analysis in a variety of cell types, providing valuable insights into its function and potential therapeutic applications. Researchers studying nuclear envelope integrity, cellular signaling pathways, and disease mechanisms can benefit from using this antibody in their experiments.
Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Purification Method:
Affinity purification
Gene ID:
2010
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Emerin/Emerin/EMD knockout (KO) 293T cells, using [KO Validated] Emerin/EMD Rabbit pAb (CAB18016) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
