The EIF2AK2 Polyclonal Antibody (CAB5578) is a valuable tool for researchers studying EIF2AK2, a key player in antiviral response pathways. This antibody, generated in rabbits, is highly specific for EIF2AK2 in human samples and has been validated for Western blot applications. By binding to the EIF2AK2 protein, this antibody allows for the detection and analysis of EIF2AK2 levels in a variety of cell types, making it a versatile tool for studies in virology and immunology.EIF2AK2, also known as protein kinase R (PKR), is a crucial component of the antiviral response, acting as a sensor for viral infection and initiating the shutdown of protein synthesis to inhibit viral replication.
Its importance in antiviral defense makes EIF2AK2 an attractive target for research into viral infections and the development of antiviral therapies. By understanding the role of EIF2AK2 in the immune response to viral infections, researchers can potentially uncover new strategies for combating a range of viral diseases.
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
Purification Method:
Affinity purification
Gene ID:
5610
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using PKR/EIF2AK2 Rabbit pAb (CAB5578) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.3s.
