The KO-Validated EGFR Polyclonal Antibody (CAB11351) is a vital tool for researchers studying the epidermal growth factor receptor (EGFR), a key player in cell growth, proliferation, and survival. This antibody, produced in rabbits, has been extensively validated for its specificity and sensitivity in various research applications, including Western blotting.EGFR is a transmembrane tyrosine kinase receptor that plays a crucial role in regulating cell growth and differentiation. Dysregulation of EGFR signaling is commonly observed in various cancers, making it an attractive target for cancer therapy. The KO-Validated EGFR Polyclonal Antibody specifically binds to EGFR, enabling researchers to detect and analyze its expression in different cell types and tissues.
With its high reactivity with human samples and reliable performance in Western blot experiments, the KO-Validated EGFR Polyclonal Antibody is an essential tool for studies in cancer biology, signal transduction, and drug development targeting the EGFR pathway. Its validation in knockout models ensures its specificity and reliability, making it a trusted reagent for researchers aiming to unravel the complexities of EGFR signaling in health and disease.
Product Name:
[KO Validated] EGFR Rabbit Polyclonal Antibody
SKU:
CAB11351
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A431 cells, using EGFR Rabbit pAb (CAB11351) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
