The EEF2K Polyclonal Antibody (CAB5404) is a valuable tool for researchers studying EEF2K, a protein kinase involved in the regulation of protein synthesis and cell proliferation. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for Western blot applications. By targeting the EEF2K protein, researchers can accurately detect and analyze its expression in various cell types, providing insights into its function in cell growth and cancer development.EEF2K, also known as eukaryotic elongation factor 2 kinase, plays a crucial role in the control of protein synthesis by regulating the elongation phase of mRNA translation.
Its dysregulation has been associated with various diseases, including cancer, making it a promising target for therapeutic interventions. By elucidating the molecular mechanisms underlying EEF2K activity, researchers can uncover novel strategies for combating cancer and other proliferative disorders.Overall, the EEF2K Polyclonal Antibody (CAB5404) holds great potential for advancing research in the fields of molecular biology, oncology, and drug discovery. Its ability to specifically bind to EEF2K enables precise detection and analysis, facilitating a deeper understanding of this key regulatory protein and its implications in disease pathology.
Product Name:
[KO Validated] EEF2K Rabbit Polyclonal Antibody
SKU:
CAB5404
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
cytoplasm, cytosol, dendritic spine, glutamatergic synapse, postsynaptic density
Calculated MW:
82kDa
Observed MW:
110kDa
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Purification Method:
Affinity purification
Gene ID:
29904
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EEF2K antibody (CAB5404) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 40s.
