The DNMT3A Rabbit Monoclonal Antibody (CAB19659) is a valuable tool for researchers studying DNA methyltransferase 3A (DNMT3A), a key enzyme involved in DNA methylation and gene regulation. This antibody, generated in rabbits, is highly specific for DNMT3A and has been validated for use in various research applications, including Western blotting.DNMT3A plays a crucial role in the establishment of DNA methylation patterns during development and in maintaining genomic stability. Dysregulation of DNMT3A has been implicated in various diseases, including cancer and neurological disorders.
By targeting DNMT3A with this antibody, researchers can investigate its function, expression levels, and localization in different cell types, providing valuable insights into its role in disease pathogenesis and potential therapeutic targeting.Overall, the DNMT3A Rabbit Monoclonal Antibody (CAB19659) is a highly specific and reliable tool for studying DNMT3A and its implications in various biological processes and disease states.
Product Name:
[KO Validated] DNMT3A Rabbit Monoclonal Antibody
SKU:
CAB19659
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human DNMT3A (Q9Y6K1).
WB,1:100 - 1:500 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
TBRS; HESJAS; DNMT3A2; M.HsaIIIA; 3A
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
102kDa
Observed MW:
85kDa/95kDa/130kDa
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Purification Method:
Affinity purification
Gene ID:
1788
Clone Number:
ARC0138
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse Thymus, using DNMT3A Rabbit mAb (CAB19659) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
