The KO Validated DNMT1 Rabbit Monoclonal Antibody (CAB19679) is a powerful tool for researchers studying DNA methyltransferase 1 (DNMT1) in various biological processes. DNMT1 is essential for maintaining DNA methylation patterns during cell division, and dysregulation of this enzyme has been implicated in numerous diseases, including cancer and neurological disorders.This antibody, specifically raised in rabbits, has been rigorously tested and validated for knockout (KO) studies, ensuring high specificity and sensitivity in detecting DNMT1 protein levels. It is suitable for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, allowing for detailed analysis of DNMT1 expression and localization in different cell types and experimental conditions.
With its ability to accurately target and detect DNMT1, this monoclonal antibody offers a valuable tool for investigating the role of DNA methylation in gene regulation, epigenetic inheritance, and disease pathogenesis. Its reliability and versatility make it an essential reagent for researchers interested in unraveling the complexity of epigenetic mechanisms and their implications for human health.
Product Name:
[KO Validated] DNMT1 Rabbit Monoclonal Antibody
SKU:
CAB19679
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_001370.1).
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1786
Clone Number:
ARC51348
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KD Validated] DNMT1 Rabbit mAb (CAB19679) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 30 s.
