The KO Validated DNAJC2 Rabbit Polyclonal Antibody (CAB19954) is a valuable tool for research involving DNAJC2, a co-chaperone protein involved in protein folding and quality control within the cell. This antibody, produced in rabbits, exhibits high reactivity with DNAJC2 in human samples and has been validated for Western blot applications. By binding specifically to the DNAJC2 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential reagent for studies in molecular biology and cell biology.DNAJC2, also known as DnaJ homolog subfamily C member 2, plays a crucial role in maintaining protein homeostasis and preventing protein misfolding, which are essential for cellular functions.
Its involvement in protein quality control processes makes it a valuable target for research into neurodegenerative diseases, cancer, and other protein misfolding disorders. Understanding the functions of DNAJC2 is key to unraveling the mechanisms underlying these diseases and developing potential therapeutic strategies to target protein-folding pathways.
Product Name:
[KO Validated] DNAJC2 Rabbit Polyclonal Antibody
SKU:
CAB19954
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human DNAJC2 (NP_055192.1).
This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
27000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and DNAJC2 knockout (KO) 293T cells, using [KO Validated] DNAJC2 Rabbit pAb (CAB19954) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
