The KO Validated DNAJB1 Polyclonal Antibody (CAB5504) is a powerful tool for researchers studying DNAJB1, a protein involved in cellular stress response and protein folding. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to DNAJB1, this antibody enables accurate detection and analysis of the protein in various cell types, making it a valuable resource for studies in molecular biology and stress response mechanisms.
DNAJB1, also known as a heat shock protein, plays a crucial role in maintaining protein homeostasis and preventing cellular damage caused by stressors such as heat, toxins, or UV radiation. Its involvement in protein folding processes and cellular stress responses makes it a key target for research into diseases like neurodegenerative disorders, cancer, and metabolic conditions. By understanding the function and regulation of DNAJB1, researchers can develop new strategies for treating and preventing these debilitating conditions.
Product Name:
[KO Validated] DNAJB1 Rabbit Polyclonal Antibody
SKU:
CAB5504
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-340 of human DNAJB1 (NP_006136.1).
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3337
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and DNAJB1 knockout (KO) 293T cells, using [KO Validated] DNAJB1 Rabbit pAb (CAB5504) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
