The KO Validated DNAJA1 Polyclonal Antibody (CAB19865) is a valuable tool for researchers studying the DNAJA1 protein, a member of the Hsp40 family of molecular chaperones. This antibody, produced in rabbits, is highly specific and sensitive for use in Western blot applications, providing reliable detection and analysis of DNAJA1 in various cell types.DNAJA1, also known as DnaJ homolog subfamily A member 1, is involved in protein folding, unfolding, and translocation processes within the cell. It plays a vital role in maintaining protein homeostasis and cellular function under stress conditions.
Research on DNAJA1 is essential for understanding its functions in physiological processes and disease states, including neurodegenerative disorders, cancer progression, and metabolic diseases.By targeting DNAJA1 with this validated antibody, researchers can explore the mechanisms by which this chaperone protein contributes to cell health and disease pathology. The antibody's high specificity and sensitivity make it an ideal tool for advancing knowledge in the fields of molecular biology, biochemistry, and biomedical research.
Product Name:
[KO Validated] DNAJA1 Rabbit Polyclonal Antibody
SKU:
CAB19865
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1).
This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
Purification Method:
Affinity purification
Gene ID:
3301
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit pAb (CAB19865) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
