The KO-Validated DDIT4 Polyclonal Antibody (CAB8086) is a powerful tool for researchers studying the role of DDIT4, a key protein involved in cellular stress response and autophagy regulation. This antibody, produced against DDIT4 in rabbits, is specifically validated for knockout (KO) studies and demonstrated to have high specificity and sensitivity in Western blot applications.DDIT4, also known as REDD1, is a critical factor in the cellular response to nutrient deprivation, hypoxia, and other stress conditions. By binding to DDIT4, this polyclonal antibody allows for precise detection and quantification of DDIT4 expression in various cell types, enabling researchers to explore its role in cellular metabolism, signal transduction, and disease processes.
Given the importance of DDIT4 in regulating cell survival and metabolism, this antibody is particularly valuable for investigations into cancer biology, metabolic disorders, and neurodegenerative diseases. By targeting DDIT4 with this validated antibody, researchers can gain crucial insights into the mechanisms underlying these conditions and develop novel therapeutic strategies targeting DDIT4.
Product Name:
[KO Validated] DDIT4 Rabbit Polyclonal Antibody
SKU:
CAB8086
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
Predicted to enable 14-3-3 protein binding activity. Involved in defense response to virus; negative regulation of TOR signaling; and response to hypoxia. Located in cytosol.
Purification Method:
Affinity purification
Gene ID:
54541
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using DDIT4 antibody (CAB8086) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
