The K.O. Validated Cyclin D1 Rabbit Monoclonal Antibody (CAB19038) is a cutting-edge tool for researchers studying the cell cycle and cancer biology. This antibody specifically targets Cyclin D1, a key regulator of cell division and proliferation. Raised in rabbits and validated for knockout (K.O.) specificity, this antibody is highly reactive with human samples and is ideal for use in Western blot applications. Its ability to bind to Cyclin D1 protein allows for precise detection and analysis in a variety of cell types.Cyclin D1 is known to play a crucial role in the development and progression of various cancers, making it a promising target for research in oncology.
By understanding the mechanisms by which Cyclin D1 functions, researchers can gain valuable insights into tumor growth and potential therapeutic strategies.Overall, the K.O. Validated Cyclin D1 Rabbit Monoclonal Antibody (CAB19038) is an indispensable tool for scientists investigating the molecular pathways involved in cell cycle regulation and cancer development. Its high specificity and reliability make it a valuable asset in advancing our understanding of cancer biology and developing targeted therapies.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Purification Method:
Affinity purification
Gene ID:
595
Clone Number:
ARC0300
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using [KO Validated] Cyclin D1 Rabbit mAb (CAB19038) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
