The CTCF Polyclonal Antibody (CAB18627) is a valuable tool for research involving CTCF, a highly conserved transcription factor known for its role in gene regulation and chromatin organization. This rabbit-derived antibody is specifically designed for use in immunohistochemistry and Western blot applications, providing reliable and specific detection of CTCF protein in various samples.CTCF is involved in the insulation of chromatin domains, enhancer-blocking activity, and transcriptional activation or repression, making it a key player in the regulation of gene expression. Its dysregulation has been implicated in various diseases, including cancer and developmental disorders.
By targeting CTCF with this validated antibody, researchers can gain insights into its function and potential implications in disease pathology.Whether studying gene regulation, chromatin structure, or disease mechanisms, the CTCF Polyclonal Antibody (CAB18627) offers a reliable tool for exploring the role of CTCF in biological processes. Its high specificity and sensitivity make it a valuable asset for researchers in the fields of genetics, epigenetics, and cancer biology.
Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
10664
Uniprot:
P49711
Cellular Location:
Chromosome, Nucleus, centromere, nucleoplasm
Calculated MW:
45kDa/82kDa
Observed MW:
140kDa
Western blot analysis of extracts from normal (control) and CTCF knockout (KO) 293T cells, using CTCF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CTCF Rabbit pAb at dilution of 1:250 (40x lens).
Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody at a dilition of 1:500.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
![Western blot - [KO Validated] CTCF Rabbit pAb](https://abclonal.com/Uploads/Catalog/A18627/A18627_1.jpg)
![Western blot - [KO Validated] CTCF Rabbit pAb](https://abclonal.com/Uploads/Catalog/A18627/A18627_2.jpg)
![Immunohistochemistry - [KO Validated] CTCF Rabbit pAb](https://abclonal.com/Uploads/Catalog/A18627/A18627_3.jpg)
![Immunoprecipitation - [KO Validated] CTCF Rabbit pAb](https://abclonal.com/Uploads/Catalog/A18627/A18627_4.jpg)
![Chromatin Immunoprecipitation - [KO Validated] CTCF Rabbit pAb](https://abclonal.com/Uploads/Catalog/A18627/A18627_5.jpg)
