The CTCF Monoclonal Antibody (CAB19588) is a powerful tool for researchers studying CTCF, a critical protein involved in gene regulation and chromatin organization. This antibody, derived from rabbits, demonstrates high specificity and sensitivity in detecting CTCF in various experimental settings, such as Western blot analysis.CTCF is a versatile transcription factor that plays a crucial role in diverse cellular processes, including DNA looping, gene expression control, and genomic imprinting. Its dysregulation has been linked to various diseases, including cancer and developmental disorders.
By targeting CTCF with this monoclonal antibody, researchers can gain valuable insights into its function and potential therapeutic applications in these disease contexts.Overall, the CTCF Monoclonal Antibody (CAB19588) is a reliable tool for researchers interested in unraveling the complexities of gene regulation and chromatin dynamics, making it an essential component for studies in genomics, epigenetics, and molecular biology.
Product Name:
[KO Validated] CTCF Rabbit Monoclonal Antibody
SKU:
CAB19588
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 628-727 of human CTCF (P49711).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 10μg-15μg of ChromatinCUT&Tag, 10⁵ cells /2 μgChIP-seq,1:50 - 1:100
Synonyms:
MRD21; FAP108; CFAP108; CTCF
Positive Sample:
Jurkat, HeLa, Mouse lung, 293T
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus, centromere, nucleoplasm.
Calculated MW:
83kDa
Observed MW:
140kDa
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10664
Clone Number:
ARC0067
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 105K562 cells with 2 ug CTCF Rabbit mAb，along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of CTCF in representative gene loci (MYC), as shown in figure.
