[KO Validated] CTCF Rabbit Monoclonal Antibody (CAB19588)
- SKU:
- CAB19588
- Product Type:
- Antibody
- Applications:
- WB
- IF
- IP
- ChIP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Product Name: | [KO Validated] CTCF Rabbit Monoclonal Antibody |
SKU: | CAB19588 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 628-727 of human CTCF (P49711). |
Sequence: | PAVE IEPE PEPQ PVTP APPP AKKR RGRP PGRT NQPK QNQP TAII QVED QNTG AIEN IIVE VKKE PDAE PAEG EEEE AQPA ATDA PNGD LTPE MILS MMDR |
Tested Applications: | WB IF/ICC IP ChIP ChIP-seq ELISA CUT&Tag |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 10μg-15μg of ChromatinCUT&Tag, 10⁵ cells /2 μgChIP-seq,1:50 - 1:100 |
Synonyms: | MRD21; FAP108; CFAP108; CTCF |
Positive Sample: | Jurkat, HeLa, Mouse lung, 293T |
Conjugate: | Unconjugated |
Cellular Localization: | Chromosome, Nucleus, centromere, nucleoplasm. |
Calculated MW: | 83kDa |
Observed MW: | 140kDa |
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method: | Affinity purification |
Gene ID: | 10664 |
Clone Number: | ARC0067 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |