[KO Validated] CSPG4 Rabbit Polyclonal Antibody (CAB3592)
- SKU:
- CAB3592
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cardiovascular
Frequently bought together:
Description
| Product Name: | [KO Validated] CSPG4 Rabbit Polyclonal Antibody |
| SKU: | CAB3592 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2). |
| Sequence: | LGAP LEGV LVEL EVLP AAIP LEAQ NFSV PEGG SLTL APPL LRVS GPYF PTLL GLSL QVLE PPQH GALQ KEDG PQAR TLSA FSWR MVEE QLIR YVHD GSET LTDS FVLM ANAS EMDR QSHP VAFT VTVL PVND QPPI LTTN TGLQ MWEG ATAP IPAE ALRS TDGD SGSE DLVY TIEQ PSNG RVVL RGAP GTEV RSFT QAQL DGGL VLFS HRGT LDGG FRFR LSDG |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
| Synonyms: | NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG; G4 |
| Positive Sample: | HeLa,A375,Mouse heart,Rat brain,Rat heart |
| Conjugate: | Unconjugated |
| Cellular Localization: | Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane. |
| Calculated MW: | 251kDa |
| Observed MW: | 250kDa-450kDa |
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
| Purification Method: | Affinity purification |
| Gene ID: | 1464 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from A375 cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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