The KO-Validated CSPG4 Polyclonal Antibody (CAB3592) is a valuable research tool for studying CSPG4, a cell surface proteoglycan involved in various biological processes. The antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the CSPG4 protein, this antibody enables precise detection and analysis in different cell types, making it ideal for research in neuroscience, cancer biology, and developmental biology.CSPG4, also known as NG2, is a key player in cell-matrix interactions, cell signaling pathways, and cell proliferation. Its involvement in neural development, tumor progression, and wound healing make it a versatile target for investigating a wide range of physiological and pathological processes.
Understanding the function of CSPG4 is essential for advancing research in areas such as neurobiology, oncology, and regenerative medicine.With its specificity and reliability, the KO-Validated CSPG4 Polyclonal Antibody (CAB3592) offers researchers a valuable tool for exploring the diverse roles of CSPG4 in health and disease. Whether studying neural stem cells, cancer cells, or tissue regeneration, this antibody provides accurate and consistent results to support groundbreaking discoveries in biomedical research.
Product Name:
[KO Validated] CSPG4 Rabbit Polyclonal Antibody
SKU:
CAB3592
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2).
Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane.
Calculated MW:
251kDa
Observed MW:
250kDa-450kDa
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Purification Method:
Affinity purification
Gene ID:
1464
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A375 cells, using [KO Validated] NG2/CSPG4 Rabbit pAb (CAB3592) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
