The KO Validated Crk-like Protein Polyclonal Antibody (CAB11735) is a powerful tool for researchers studying Crk-like proteins, important signaling molecules involved in various cellular processes such as cell adhesion, migration, and apoptosis. This antibody, generated in rabbits, is highly specific for detecting the Crk-like protein in human samples and has been validated for use in Western blot applications.The Crk-like protein, a member of the Crk family of adaptor proteins, plays a crucial role in mediating signaling pathways that regulate cell morphology and motility. Dysregulation of Crk-like protein expression has been implicated in various diseases, including cancer and developmental disorders.
By using the CAB11735 antibody, researchers can accurately detect and analyze the levels of Crk-like protein in different cell types, providing valuable insights into its function and potential involvement in disease pathogenesis.Furthermore, the validation of this antibody for knockout (KO) studies ensures its specificity and reliability, allowing researchers to confidently explore the biological functions of Crk-like proteins in both normal and disease states. This antibody is a valuable tool for investigations in cell biology, cancer research, and drug development, offering new possibilities for understanding and targeting Crk-like proteins in therapeutic applications.
Product Name:
[KO Validated] CRKL Rabbit Polyclonal Antibody
SKU:
CAB11735
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).
cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm
Calculated MW:
34kDa
Observed MW:
37kDa
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Purification Method:
Affinity purification
Gene ID:
1399
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CRKL Antibody (CAB11735) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
