The CDKN2C (p18 INK4C) Rabbit Polyclonal Antibody (CAB18054) is a valuable tool for research involving CDKN2C, a tumor suppressor gene that plays a crucial role in cell cycle regulation and tumor development. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it suitable for use in various applications such as immunohistochemistry and western blotting.CDKN2C, also known as p18 INK4C, functions as a negative regulator of cell cycle progression by inhibiting cyclin-dependent kinases and preventing cell division. Its role in tumor suppression makes it a key target for studies in cancer research, with potential implications for understanding cancer development and therapeutic interventions.
The validation of this antibody for use in western blot applications ensures reliable and accurate detection of CDKN2C protein levels in cell lysates, tissue samples, and other biological samples. Its specificity and sensitivity make it an essential tool for researchers investigating the mechanisms of cell cycle control, tumor suppression, and potential therapeutic targets in cancer biology.
The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to interact with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. Ectopic expression of this gene was shown to suppress the growth of human cells in a manner that appears to correlate with the presence of a wild-type RB1 function. Studies in the knockout mice suggested the roles of this gene in regulating spermatogenesis, as well as in suppressing tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode an identical protein, have been reported.
Purification Method:
Affinity purification
Gene ID:
1031
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CDKN2C/p18-INK4C knockout (KO) HeLa cells, using [KO Validated] CDKN2C/p18-INK4C Rabbit pAb (CAB18054) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
