The CDKN1A (p21Cip1) Rabbit Monoclonal Antibody (CAB19094) is a valuable tool for researchers studying the CDKN1A protein, also known as p21Cip1, which functions as a key regulator of cell cycle progression and cell proliferation. This antibody, generated in rabbits, is highly specific for human samples and has been extensively validated for use in Western blot applications.CDKN1A is a crucial player in cellular processes such as DNA damage response, senescence, and apoptosis, making it an important target for research in cancer biology and aging. By binding to the CDKN1A protein, this antibody enables precise detection and quantification of the protein in various cell types, allowing for detailed analysis of its expression levels and subcellular localization.
The ability to study CDKN1A expression and function with this validated antibody opens up opportunities for further insights into cell cycle control, tumor suppression, and therapeutic interventions targeting abnormal cell proliferation. With its high specificity and reliability, the CDKN1A (p21Cip1) Rabbit Monoclonal Antibody (CAB19094) is an essential tool for advancing research in oncology and cell biology.
MCF7 cells,HeLa cells,293T cells,Mouse large intestine,Mouse testis,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
18kDa
Observed MW:
18kDa(Mouse)/21kDa(Human/Rat)
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1026
Clone Number:
ARC51034
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (CAB19094) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
