The CDK8 Polyclonal Antibody (CAB5548) is a valuable tool for researchers studying CDK8, a protein kinase known for its role in transcriptional regulation and cell cycle progression. This antibody, generated in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.CDK8 is a member of the cyclin-dependent kinase (CDK) family and is involved in the regulation of gene expression by phosphorylating transcription factors. Dysregulation of CDK8 has been linked to various diseases, including cancer, making it an important target for therapeutic intervention.
With the ability to detect and analyze CDK8 protein levels in different cell types, this antibody is essential for studies in cancer biology, cell cycle regulation, and transcriptional control. By understanding the function of CDK8, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets for cancer and other diseases.
Product Name:
[KO Validated] CDK8 Rabbit Polyclonal Antibody
SKU:
CAB5548
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
K35; IDDHBA; K8
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
53kDa
Observed MW:
56kDa
This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1024
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using [KO Validated] CDK8 Rabbit pAb (CAB5548) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
