The KO Validated CDK6 Polyclonal Antibody (CAB1545) is a powerful tool for researchers studying the cell cycle regulator Cyclin-Dependent Kinase 6 (CDK6). This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications.CDK6 is a key player in cell cycle regulation, promoting cell proliferation and division. Dysregulation of CDK6 has been linked to various cancers, making it a potential target for cancer therapy. By using the CAB1545 antibody, researchers can effectively detect and analyze CDK6 protein levels in different cell types, providing valuable insights into cell cycle control mechanisms.
This antibody has been rigorously validated for knockout (KO) experiments, ensuring accurate and reliable results. It is a valuable tool for studies in cancer research, cell biology, and drug development, offering researchers a deeper understanding of CDK6 function and its potential implications for disease treatment strategies. Unlock the potential of CDK6 research with the KO Validated CDK6 Polyclonal Antibody (CAB1545).
Product Name:
CDK6 Polyclonal Antibody
SKU:
CAB1545
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 226-326 of human CDK6 (NP_001250.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MCPH12; PLSTIRE; CDK6
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle.
Calculated MW:
37kDa
Observed MW:
40kDa
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.
Purification Method:
Affinity purification
Gene ID:
1021
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using CDK6 Rabbit pAb (CAB1545) at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
