The KO-Validated CDK4 Rabbit Monoclonal Antibody is a cutting-edge research tool designed for scientists studying the CDK4 protein, an essential regulator of cell cycle progression and a target for cancer therapy. This antibody is rigorously validated for knockout (KO) cells, ensuring specificity and reliability in experimental settings.Raised in rabbits, this monoclonal antibody exhibits high reactivity with human samples, making it a versatile tool for a wide range of applications, including Western blotting, immunoprecipitation, and immunofluorescence. Its ability to specifically bind to CDK4 enables accurate detection and quantification of the protein in various cellular contexts, offering valuable insights into cell proliferation, differentiation, and tumorigenesis processes.
With its proven efficacy and specificity, the KO-Validated CDK4 Rabbit Monoclonal Antibody is an indispensable asset for researchers in the fields of oncology, cell biology, and drug development. By elucidating the role of CDK4 in cancer progression and identifying potential therapeutic targets, this antibody paves the way for innovative approaches in personalized medicine and precision oncology.
Product Name:
[KO Validated] CDK4 Rabbit Monoclonal Antibody
SKU:
CAB11136
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Monkey
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 204-303 of human CDK4 (NP_000066.1).
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Purification Method:
Affinity purification
Gene ID:
1019
Clone Number:
ARC51004
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CDK4 Rabbit mAb (CAB11136) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
