The CDK2AP1 Polyclonal Antibody (CAB19985) is a valuable tool for researchers studying CDK2AP1, a protein known to play a role in cell cycle regulation and tumor suppression. This rabbit polyclonal antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to CDK2AP1, the antibody allows for the detection and analysis of this protein in various cell types, making it ideal for use in studies focused on cell proliferation, cancer biology, and cell signaling pathways.
CDK2AP1, also known as cyclin-dependent kinase 2-associated protein 1, is a key regulator of cell division and has been implicated in the development and progression of various cancers. Understanding the function and regulation of CDK2AP1 is essential for unraveling its potential as a therapeutic target in cancer treatment. Researchers studying cell cycle control, tumor suppression, and oncogenic pathways will find this antibody to be a valuable tool in their investigations.
Product Name:
[KO Validated] CDK2AP1 Rabbit Polyclonal Antibody
SKU:
CAB19985
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human CDK2AP1 (NP_004633.1).
cytoplasm, cytosol, nucleoplasm, nucleus, perinuclear region of cytoplasm
Calculated MW:
12kDa
Observed MW:
12kDa
The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
8099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit pAb (CAB19985) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
