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[KO Validated] CDK2AP1 Rabbit Polyclonal Antibody (CAB19985)

SKU:
CAB19985
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] CDK2AP1 Rabbit Polyclonal Antibody
SKU:CAB19985
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human CDK2AP1 (NP_004633.1).
Sequence:MSYK PNLA AHMP AAAL NAAG SVHS PSTS MATS SQYR QLLS DYGP PSLG YTQG TGNS QVPQ SKYA ELLA IIEE LGKE IRPT YAGS KSAM ERLK RGII HARG LVRE CLAE TERN ARS
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:DOC1; ST19; DORC1; doc-1; p12DOC-1; P1
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:cytoplasm, cytosol, nucleoplasm, nucleus, perinuclear region of cytoplasm
Calculated MW:12kDa
Observed MW:12kDa

The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification Method:Affinity purification
Gene ID:8099
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and CDK2AP1 knockout (KO) 293T cells, using [KO Validated] CDK2AP1 Rabbit pAb (CAB19985) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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