The KO Validated Caspase-3 Polyclonal Antibody (CAB2156) is a valuable tool for researchers studying caspase-3, an important enzyme involved in apoptosis, or programmed cell death. The antibody, produced in rabbits, has been rigorously tested and validated for use in knockout (KO) studies, ensuring accurate and reliable results.Caspase-3 is a key player in the apoptotic pathway and is implicated in various diseases, including cancer and neurodegenerative disorders. By targeting caspase-3 with this highly specific antibody, researchers can investigate its role in disease progression and potential as a therapeutic target.
This antibody is suitable for use in a variety of applications, including Western blotting and immunofluorescence, providing flexibility for researchers working in different experimental settings. With its high sensitivity and specificity, the KO Validated Caspase-3 Polyclonal Antibody (CAB2156) is a valuable asset for advancing our understanding of apoptosis and its implications in disease.
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Caspase-3 Rabbit pAb (CAB2156) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
