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[KO Validated] Calpain 2 Rabbit Polyclonal Antibody (CAB1861)

SKU:
CAB1861
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-CAPN2 Antibody CAB1861KO Validated
  • KO Validated Antibodies 1 Anti-CAPN2 Antibody CAB1861KO Validated
  • KO Validated Antibodies 1 Anti-CAPN2 Antibody CAB1861KO Validated
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Calpain 2 Rabbit Polyclonal Antibody
SKU:CAB1861
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-680 of human Calpain 2 (NP_001739.2).
Sequence:SDTY KKWK LTKM DGNW RRGS TAGG CRNY PNTF WMNP QYLI KLEE EDED EEDG ESGC TFLV GLIQ KHRR RQRK MGED MHTI GFGI YEVP EELS GQTN IHLS KNFF LTNR ARER SDTF INLR EVLN RFKL PPGE YILV PSTF EPNK DGDF CIRV FSEK KADY QAVD DEIE ANLE EFDI SEDD IDDG FRRL FAQL AGED AEIS AFEL QTIL RRVL AKRQ DIKS DGFS IETC KIMV DMLD SDGS GKLG LKEF YILW TKIQ KYQK IYRE IDVD RSGT MNSY EMRK ALEE AGFK MPCQ LHQV IVAR FADD QLII DFDN FVRC LVRL ETLF KIFK QLD
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:CANP2; mCANP; CANPL2; CANPml; 2
Positive Sample:HeLa,U-251MG,Mouse kidney,Mouse heart,Rat kidney
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm.
Calculated MW:80kDa
Observed MW:80kDa

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:824
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from normal (control) and Calpain 2 knockout (KO) 293T cells, using Calpain 2 antibody (CAB1861) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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