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[KO Validated] BRD4 Rabbit Polyclonal Antibody (CAB20019)

SKU:
CAB20019
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • KO Validated Antibodies 2 Anti-BRD4-S Antibody CAB20019KO Validated
  • KO Validated Antibodies 2 Anti-BRD4-S Antibody CAB20019KO Validated
$167 - $503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] BRD4 Rabbit Polyclonal Antibody
SKU:CAB20019
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600-722 of human BRD4 (NP_055114.1).
Sequence:ESEE EDKC KPMS YEEK RQLS LDIN KLPG EKLG RVVH IIQS REPS LKNS NPDE IEID FETL KPST LREL ERYV TSCL RKKR KPQA EKVD VIAG SSKM KGFS SSES ESSS ESSS SDSE DSET GPA
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CAP; MCAP; HUNK1; HUNKI; FSHRG4; D4
Positive Sample:HeLa,HeLa-BRD4-FL,HeLa-BRD4-S(a),HeLa-BRD4-S(b)
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:80kDa/88kDa/152kDa
Observed MW:200kDa/140kDa/120kDa

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.

Purification Method:Affinity purification
Gene ID:23476
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and BRD4 knockout (KO) HeLa cells, using [KO Validated] BRD4 Rabbit pAb (CAB20019) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.

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