The KO Validated Beta-Catenin Rabbit Monoclonal Antibody (CAB19657) is a cutting-edge tool for researchers studying beta-catenin, a key protein involved in cell adhesion and signaling pathways. This antibody, produced using rabbit monoclonal technology, specifically targets human beta-catenin and has been rigorously validated for use in Western blot applications.Beta-catenin plays a crucial role in cell signaling processes that regulate cell proliferation, differentiation, and migration. Dysregulation of beta-catenin is linked to various diseases, including cancer and developmental disorders. The CAB19657 antibody enables precise detection of beta-catenin protein levels in different cell types, making it a valuable asset for studies in oncology, developmental biology, and regenerative medicine.
By facilitating the analysis of beta-catenin expression and localization, researchers can gain insights into the molecular mechanisms underlying disease processes and potentially identify new therapeutic targets. The CAB19657 antibody offers high specificity and sensitivity, making it an essential tool for advancing our understanding of beta-catenin biology and its implications in health and disease.
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
EVR7; CTNNB; MRD19; NEDSDV; armadillo; in
Positive Sample:
HeLa cells,293T cells,NIH/3T3 cells,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
92kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Clone Number:
ARC0136
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using [KO Validated] β-Catenin Rabbit mAb (CAB19657) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
