The Bag2 Polyclonal Antibody (CAB19945) is a powerful tool for researchers studying Bag2, a key regulatory protein in the BAG family involved in cellular stress responses. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards Bag2 in human samples, making it an ideal choice for Western blot applications. By binding to Bag2, this antibody enables precise detection and analysis of this important protein in a variety of cell types, particularly in the fields of molecular biology and cell signaling research.Bag2 is known for its critical role in cellular proteostasis and responses to stress, making it a valuable target for investigations into diseases such as neurodegenerative disorders, cancer, and metabolic syndromes.
By understanding the function and regulation of Bag2, researchers can gain insights into potential therapeutic targets and mechanisms for combating these diseases. The Bag2 Polyclonal Antibody is a reliable tool for exploring the intricate pathways involving Bag2 and its impact on cellular physiology and disease progression.
Product Name:
[KO Validated] BAG2 Rabbit Polyclonal Antibody
SKU:
CAB19945
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1).
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Purification Method:
Affinity purification
Gene ID:
9532
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and BAG2 knockout (KO) HeLa cells, using [KO Validated] BAG2 Rabbit pAb (CAB19945) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
