The KO Validated ATG5 Rabbit Monoclonal Antibody (CAB19677) is a cutting-edge research tool designed for the study of ATG5, a key player in the autophagy process. This antibody, produced in rabbits, demonstrates high specificity and sensitivity in detecting ATG5 protein in a variety of samples, including human tissues. Validated for use in Western blot applications, this monoclonal antibody offers researchers a reliable tool for analyzing ATG5 expression levels and localization in different cell types. Autophagy, the cellular process of degrading and recycling components, is a crucial mechanism for maintaining cellular homeostasis and responding to stressors such as nutrient deprivation and infection.
By targeting ATG5, researchers can gain valuable insights into the regulation and function of autophagy in various physiological and pathological conditions. This antibody is an essential resource for studies in cell biology, cancer research, and neurodegenerative diseases, where dysregulation of autophagy has been linked to disease progression. Unlock the potential of autophagy research with the KO Validated ATG5 Rabbit Monoclonal Antibody (CAB19677).
Product Name:
[KO Validated] ATG5 Rabbit Monoclonal Antibody
SKU:
CAB19677
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ATG5 (Q9H1Y0).
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
9474
Clone Number:
ARC0156
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and ATG5 knockout (KO) 293T cells, using [KO Validated] ATG5 Rabbit mAb (CAB19677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
