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[KO Validated] ATG4D Rabbit Polyclonal Antibody (CAB18097)

SKU:
CAB18097
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] ATG4D Rabbit Polyclonal Antibody
SKU:CAB18097
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ATG4D (NP_116274.3).
Sequence:MNSV SPAA AQYR SSSP EDAR RRPE ARRP RGPR GPDP NGLG PSGA SGPA LGSP GAGP SEPD EVDK FKAK FLTA WNNV KYGW VVKS RTSF SKIS SIHL CGRR YRFE GEGD IQRF QRDF VSRL WLTY RRDF PPLP GGCL TSDC GWGC MLRS GQMM LAQG LLLH FLPR DWTW AEGM GLGP PELS GSAS PSRY HGPA RWMP PRWA QGAP ELEQ ERRH RQIV SWFA DHPR APFG LH
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:APG4D; AUTL4; APG4-D; HsAPG4D; 4D
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Mitochondrion matrix.
Calculated MW:53kDa
Observed MW:53kDa

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:84971
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit pAb (CAB18097) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

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