The ARHGEF1 Polyclonal Antibody (CAB4274) is a research tool designed for the study of ARHGEF1, a key regulator of cellular processes such as cell migration, adhesion, and signaling. This antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in Western blot applications. By binding specifically to the ARHGEF1 protein, this antibody allows for the visualization and analysis of ARHGEF1 expression in various cell types.ARHGEF1, also known as Rho guanine nucleotide exchange factor 1, is involved in the activation of Rho GTPases, which play essential roles in cytoskeletal organization and cell motility.
Dysregulation of ARHGEF1 has been implicated in cancer progression, making it a promising target for cancer research. Additionally, ARHGEF1 has been linked to immune system function and neurological disorders, highlighting its significance in various biological processes.Research on ARHGEF1 is crucial for gaining insights into its role in normal cellular function and disease pathogenesis. By using the ARHGEF1 Polyclonal Antibody (CAB4274), researchers can further elucidate the mechanisms underlying ARHGEF1-mediated cellular processes, contributing to the development of targeted therapies for cancer and other diseases.
Product Name:
[KO Validated] ARHGEF1 Rabbit Polyclonal Antibody
SKU:
CAB4274
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Purification Method:
Affinity purification
Gene ID:
9138
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] ARHGEF1 Rabbit pAb (CAB4274) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
