Anti-ARF1 Antibody (CAB19977)[KO Validated]
- SKU:
- CAB19977
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-ARF1 Antibody (CAB19977)[KO Validated]
The KO-validated ARF1 Rabbit Polyclonal Antibody (CAB19977) is a valuable tool for researchers studying ARF1, a small GTPase protein essential for membrane trafficking and organelle structure regulation. This antibody, generated in rabbits, exhibits high reactivity with ARF1 in knockout samples and has been validated for use in Western blot applications. By specifically binding to ARF1, this antibody enables precise detection and analysis of the protein in various cellular contexts, making it an ideal choice for investigations in cell biology and membrane dynamics.ARF1, a key regulator of vesicle formation and transport, plays a crucial role in maintaining cellular homeostasis and proper organelle function.
Dysregulation of ARF1 has been implicated in various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic intervention. Understanding the functions and mechanisms of ARF1 is essential for developing potential treatments that target membrane trafficking pathways and organelle dynamics.Overall, the KO-validated ARF1 Rabbit Polyclonal Antibody (CAB19977) offers researchers a reliable tool for studying the intricate roles of ARF1 in cellular processes and disease pathogenesis, paving the way for advancements in drug discovery and novel therapeutic strategies.
| Antibody Name: | Anti-ARF1 Antibody [KO Validated] |
| Antibody SKU: | CAB19977 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human ARF1. |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant protein of human ARF1. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 375 |
| Uniprot: | P84077 |
| Cellular Location: | Cell junction, Cytoplasm, Golgi apparatus, Lipid-anchor, Membrane, perinuclear region, postsynaptic cell membrane, postsynaptic density, synapse, synaptosome |
| Calculated MW: | 20kDa |
| Observed MW: | 18KDa |
![Anti-ARF1 Antibody [KO Validated] (CAB19977)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/x/948/A19977_1__57988.jpg) | Western blot analysis of extracts from normal (control) and ARF1 knockout (KO) HeLa cells, using ARF1 antibody (CAB19977) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
| Synonyms: | ARF1 |
| Background: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric Chromosomal Location of Human Ortholog: 1q42 Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol Molecular Function:GTPase activity; protein binding; GTP binding; receptor signaling protein activity Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus |
| NCBI Summary: | ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P84077 |
| NCBI GenInfo Identifier: | 51316985 |
| NCBI Gene ID: | 375 |
| NCBI Accession: | P84077.2 |
| UniProt Secondary Accession: | P84077,P10947, P32889, |
| UniProt Related Accession: | P84077 |
| Molecular Weight: | 181 |
| NCBI Full Name: | ADP-ribosylation factor 1 |
| NCBI Synonym Full Names: | ADP-ribosylation factor 1 |
| NCBI Official Symbol: | ARF1Â Â |
| NCBI Protein Information: | ADP-ribosylation factor 1 |
| UniProt Protein Name: | ADP-ribosylation factor 1 |
| Protein Family: | Auxin response factor |
| UniProt Gene Name: | ARF1Â Â |
| UniProt Entry Name: | ARF1_HUMAN |
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