[KO Validated] APP Rabbit Monoclonal Antibody (CAB17911)
- SKU:
- CAB17911
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
| Product Name: | [KO Validated] APP Rabbit Monoclonal Antibody |
| SKU: | CAB17911 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 671-770 of human APP (P05067). |
| Sequence: | MDAE FRHD SGYE VHHQ KLVF FAED VGSN KGAI IGLM VGGV VIAT VIVI TLVM LKKK QYTS IHHG VVEV DAAV TPEE RHLS KMQQ NGYE NPTY KFFE QMQN |
| Tested Applications: | WB IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma; alpha-sAPP; PP |
| Positive Sample: | 293T,HeLa,U-87 MG,Mouse brain,Rat brain |
| Conjugate: | Unconjugated |
| Cellular Localization: | Membrane, Single-pass type I membrane protein, clathrin-coated pit. |
| Calculated MW: | 87kDa |
| Observed MW: | 100-140kDa |
This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 351 |
| Clone Number: | ARC0465 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from wild type (WT) and APP knockout (KO) 293T cells, using [KO Validated] APP Rabbit mAb (CAB17911) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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