[KO Validated] AKT2 Rabbit Polyclonal Antibody (CAB18019)
- SKU:
- CAB18019
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- ICC
- IF
- IP
- ChIP
- Reactivity:
- Human
- Mouse
- Rat
- Monkey
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Product Name: | [KO Validated] AKT2 Rabbit Polyclonal Antibody |
| SKU: | CAB18019 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 381-481 of human AKT2 (NP_001617.1). |
| Sequence: | LAGL LKKD PKQR LGGG PSDA KEVM EHRF FLSI NWQD VVQK KLLP PFKP QVTS EVDT RYFD DEFT AQSI TITP PDRY DSLG LLEL DQRT HFPQ FSYS ASIR E |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:1000 - 1:5000 |
| Synonyms: | PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; AKT2 |
| Positive Sample: | 293T,Raji,Jurkat,HeLa,LO2,Mouse kidney |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein. |
| Calculated MW: | 56kDa |
| Observed MW: | 60kDa |
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
| Purification Method: | Affinity purification |
| Gene ID: | 208 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using AKT2 Rabbit pAb (CAB18019) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s. |
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