[KO Validated] active + pro Caspase-3 Rabbit Monoclonal Antibody (CAB19654)
The KO-Validated Active Pro-Caspase-3 Rabbit Monoclonal Antibody (CAB19654) is a cutting-edge tool for researchers studying the caspase-3 protein, a key player in the process of programmed cell death (apoptosis). This antibody, developed using rabbit monoclonal technology, is highly specific and sensitive for detecting active pro-caspase-3 in various biological samples.Validated for knockout (KO) studies, this antibody is ideal for confirming the presence and activity of pro-caspase-3 in cell lysates or tissue samples. Its high specificity ensures accurate results in Western blot applications, making it an essential tool for researchers investigating apoptosis, cell signaling pathways, and potential therapeutic targets in cancer research and other disease contexts.
By using the KO-Validated Active Pro-Caspase-3 Rabbit Monoclonal Antibody (CAB19654), researchers can gain valuable insights into the regulation of apoptosis and potential mechanisms of cell death in normal and diseased states. This antibody is a reliable and efficient tool for advancing scientific understanding and developing novel strategies for treating conditions related to abnormal cell survival and death pathways.
Product Name:
[KO Validated] active + pro Caspase-3 Rabbit Monoclonal Antibody
SKU:
CAB19654
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (P42574).
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Clone Number:
ARC0133
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] active + pro Caspase-3 Rabbit mAb (CAB19654) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
