The KO Validated MCM6 Monoclonal Antibody is a cutting-edge tool for researchers studying the cell cycle and DNA replication processes. This monoclonal antibody, specifically designed for use in knockout studies, is highly specific to the MCM6 protein, a key component of the DNA replication licensing machinery.The antibody, rigorously tested and validated in knockout cell lines, is ideal for Western blotting and immunofluorescence applications in a variety of cell types. Its exceptional specificity and sensitivity make it a valuable asset for investigations into cell cycle regulation, DNA replication dynamics, and mechanisms of genomic stability.
By targeting MCM6, researchers can gain valuable insights into the intricate processes of DNA replication and cell proliferation. This antibody is a valuable tool for advancing our understanding of fundamental biological processes and potential therapeutic targets in cancer and other diseases characterized by dysregulated cell division.
Product Name:
[KO/KD Validated] MCM6 Monoclonal Antibody
SKU:
CAB21112
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 601-821 of human MCM6 (NP_005906.2).
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.
Purification Method:
Affinity purification
Gene ID:
4175
Clone Number:
ARC3057
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KD Validated] MCM6 Rabbit mAb (CAB21112) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
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