The KLK3 Polyclonal Antibody (CAB2052) is a valuable tool for researchers studying the KLK3 protein, also known as prostate-specific antigen (PSA). This antibody is produced in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By binding specifically to the KLK3 protein, this antibody enables the detection and analysis of KLK3 in various cell types.The KLK3 protein is primarily known for its role as a biomarker for prostate cancer, where elevated levels of PSA in the blood can indicate the presence of the disease.
However, KLK3 is also involved in other physiological processes, such as semen liquefaction and male fertility. Understanding the regulation and function of KLK3 is important for advancing research in prostate cancer diagnostics and treatment.Overall, the KLK3 Polyclonal Antibody (CAB2052) provides a reliable tool for researchers interested in studying the KLK3 protein and its implications in prostate cancer and other related research areas. Its high reactivity and specificity make it a valuable asset for immunology and cancer research studies.
Product Name:
KLK3 Rabbit Polyclonal Antibody
SKU:
CAB2052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-261 of human KLK3 (NP_001639.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
APS; PSA; hK3; KLK2A1; KLK3
Positive Sample:
LNCaP
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
29kDa
Observed MW:
34kDa
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease which is synthesized in the epithelial cells of the prostate gland, and is present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. The serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
354
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from LNCaP cells, using KLK3 Rabbit pAb (CAB2052) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
